Elena Zaikova, Alyse Hawley, David A. Walsh, Steven J. Hallam
This video documents methods for collecting coastal marine water samples and processing them for various downstream applications including biomass concentration, nucleic acid purification, cell abundance, nutrient and trace gas analyses.
Jody J. Wright, Sangwon Lee, Elena Zaikova, David A. Walsh, Steven J. Hallam
We describe a method for extraction of high molecular weight genomic DNA from planktonic biomass concentrated on 0.22 μm Sterivex filters, followed by cesium chloride density gradient centrifugation for purification.
Marcus Taupp, Sangwon Lee, Alyse Hawley, Jinshu Yang, Steven J. Hallam
Construction of a fosmid library with environmental genomic DNA isolated from the vertical depth continuum of a seasonally hypoxic fjord is described. The resulting clone library is picked into 384-well plates and archived for downstream sequencing and functional screening by the application of an automated colony picking system.